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Induction of Maternal Tolerance to Fetal Alloantigens by RANTES Production
Author(s) -
Ramhorst Rosanna,
Patel Rahdika,
Corigliano Adriana,
Etchepareborda Juan José,
Fainboim Leonardo,
Schust Danny
Publication year - 2006
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/j.1600-0897.2006.00430.x
Subject(s) - cd8 , immune system , t cell , chemokine , biology , flow cytometry , pregnancy , immunology , medicine , endocrinology , genetics
Problem Previous studies have demonstrated a requirement for RANTES (regulated on activated normal T‐cell expressed, and secreted) at immune privileged sites; we have investigated the role of RANTES in the induction of maternal–fetal tolerance. Method of study Endometrial and peripheral T lymphocytes were obtained from women with recurrent pregnancy losses (RPLs) and fertile women. RANTES modulation by progesterone or paternal alloantigens was measured by enzyme‐linked immunosorbent assay or flow cytometry analysis. Results Progesterone significantly increased intracellular RANTES expression in CD4 + and CD8 + endometrial T cells. Moreover, alloreactive lymphocytes from RPL patients produced lower RANTES levels when compared with those from fertile women. At the local level, treatment with recombinant RANTES induced a decrease in CCR5 and CXCR4 messenger RNA that correlated with an increase in T‐bet expression. RPL patients and normally fertile women express RANTES similarly, but differ in their patterns of RANTES receptor expression. Conclusion RANTES may be implicated in the local induction of a Th1‐type response necessary for successful implantation. Altered response to RANTES stimulation among some RPL patients may be responsible for poor pregnancy outcomes.