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Adoptive Transfer of Paternal Antigen‐Hyporesponsive T Cells Facilitates a Th2 Bias in Peripheral Lymphocytes and at Materno‐Fetal Interface in Murine Abortion‐prone Matings
Author(s) -
Jin LiPing,
Zhou YueHua,
Zhu XiaoYong,
Wang MingYan,
Li DaJin
Publication year - 2006
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/j.1600-0897.2006.00425.x
Subject(s) - adoptive cell transfer , cytokine , antigen , immunology , biology , fetus , monoclonal antibody , andrology , t cell , endocrinology , antibody , medicine , pregnancy , immune system , genetics
Problem To investigate the Th1/Th2 cytokine changes in abortion‐prone recipient mice adoptively transferred by the paternal antigen‐hyporesponsive T cells. Method of study The paternal antigen‐hyporesponsive T cells were generated by the anti‐B7 monoclonal antibody (mAb) treatment and adoptively transferred into pregnant CBA/J mice of abortion‐prone matings on day 4 of gestation. The intracellular expressions of Th1 cell‐derived cytokine, tumor necrosis factor‐ α , γ ‐interferon and interleukin‐2 (IL‐2) and Th2 cell‐derived cytokine, IL‐4 and IL‐10 in the maternal spleen were analyzed by flow cytometry, and secretions of the Th1 and Th2 cytokines in supernatant of the feto‐placental unit culture were analyzed by an enzyme‐linked immunosorbent assay. Results Our findings showed the increased secretion of Th1 cytokines and the decreased secretion of Th2 cytokines in abortion‐prone matings. Treatment with anti‐B7 mAbs on day 4 of gestation enhanced Th2 and reduced Th1 cytokine production in abortion‐prone matings. Similarly, adoptive transfer of paternal antigen‐hyporesponsive T cells induced maternal tolerance to the fetus and displayed a Th2 bias both in the peripheral lymphocytes and at the materno‐fetal interface of the abortion‐prone matings. Conclusions These findings indicate that the Th2 cytokine bias and an increase in fetal viability induced by the anti‐B7 mAb treatment can be transferred to other pregnant mice of the abortion‐prone matings.