Premium
Human Leukocyte Antigen F Protein is Expressed in the Extra‐Villous Trophoblasts but Not on the Cell Surface of them
Author(s) -
Nagamatsu Takeshi,
Fujii Tomoyuki,
Matsumoto Junko,
Yamashita Takahiro,
Kozuma Shiro,
Taketani Yuji
Publication year - 2006
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/j.1600-0897.2006.00414.x
Subject(s) - human leukocyte antigen , antigen , immunohistochemistry , biology , hla g , microbiology and biotechnology , placenta , immunology , cell , antibody , virology , fetus , pregnancy , genetics
Problem Human leukocyte antigen (HLA)‐F together with other class 1b HLAs may play a pivotal role in immunological interaction at feto‐maternal interface. However, it is not elucidated whether HLA‐F is expressed on cell surface of extra‐villous trophoblasts (EVTs). Method of study The localization of HLA‐F in placenta of each trimester was investigated by immunohistochemistry. The expression modality of HLA‐F molecule was compared with that of HLA‐G in cultured EVTs using flowcytometry. Results and conclusion In immunohistochemical study, remarkable staining for HLA‐F was confirmed in EVTs throughout gestation. HLA‐F expression was restricted to be intracellular in cultured EVTs. HLA‐F expression was increased with time in culture, which was accompanied by the increase of cell surface HLA‐G. Consequently, HLA‐F lacking in cell surface expression on EVTs may not contribute to the direct cell‐to‐cell immune reaction but might work supportively modifying the function of HLA‐G.