Premium
Granulocyte‐Macrophage Colony‐Stimulating‐Factor Increases Interferon‐ τ Protein Secretion in Bovine Trophectoderm Cells
Author(s) -
Michael Donna D.,
Wagner Sara K.,
Ocón Olga M.,
Talbot Neil C.,
Rooke John A.,
Ealy Alan D.
Publication year - 2006
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/j.1600-0897.2006.00390.x
Subject(s) - conceptus , granulocyte macrophage colony stimulating factor , secretion , biology , colony stimulating factor , andrology , granulocyte , cytokine , endocrinology , medicine , interferon , interferon gamma , immunology , pregnancy , fetus , microbiology and biotechnology , haematopoiesis , stem cell , genetics
Problem Uterine‐derived factors are required for optimal conceptus development and secretion of the maternal recognition of pregnancy factor, interferon‐ τ (IFN‐ τ ). Identifying these factors may lead to the development of schemes for increasing pregnancy success in cattle. Method of Study The objectives were to examine the effects of granulocyte‐macrophage colony‐stimulating‐factor (GM‐CSF) on trophectoderm proliferation rates and IFN‐ τ production, and verify the appropriateness of using an in vitro model of bovine trophectoderm (CT‐1 cell). Results Rate of [ 3 H]‐thymidine incorporation into DNA was increased by supplementation of CT‐1 medium with 10 or 100 ng/mL porcine (po) GM‐CSF. GM‐CSF supplementation to CT‐1 medium also increased IFN‐ τ secretion. When results were normalized to account for number of CT‐1 cells, 10 and 100 ng/mL poGM‐CSF increased antiviral activity and IFN‐ τ concentrations (using an IFN‐ τ ‐specific enzyme‐linked immunosorbent assay) in CT‐1 conditioned medium compared with controls. Conclusions These findings indicate that GM‐CSF increases proliferation and IFN‐ τ production in bovine trophectoderm.