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Neutrophil Function During Pregnancy: Is Nitric Oxide Production Correlated with Superoxide Production?
Author(s) -
Tsukimori Kiyomi,
Fukushima Kotaro,
Komatsu Hajime,
Nakano Hitoo
Publication year - 2006
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/j.1600-0897.2005.00324.x
Subject(s) - nitric oxide , superoxide , nitrite , superoxide dismutase , pregnancy , chemotaxis , neutrophile , medicine , chemistry , andrology , endocrinology , immunology , biochemistry , biology , inflammation , enzyme , nitrate , receptor , organic chemistry , genetics
Problem Oxygen radical formation by neutrophils during pregnancy is not well studied. Method of study We studied neutrophil‐derived superoxide anion (O) and nitric oxide (NO) values in 75 normal pregnant women, 12 postpartum women, and 10 non‐pregnant women. O production was measured by the superoxide dismutase‐inhibitable reduction of ferricytochrome c . NO production was measured by accumulation of the stable end product nitrite using a modified Griess reaction method. Results O production of neutrophils stimulated by chemotactic peptide was significantly enhanced in the early second trimester of pregnancy. l ‐arginine analogue‐inhibitable nitrite production was induced in neutrophils from pregnant women, but not from postpartum and non‐pregnant subjects. In third‐trimester subjects but not non‐pregnant subjects, neutrophils pre‐treated with l ‐arginine analogues enhanced O production compared with untreated neutrophils. Conclusion These findings indicate that O and NO production by neutrophils during pregnancy were modulated separately, whereas neutrophil‐derived NO might function as a regulator of O.