Premium
Comparison of T Cell Subsets Between Somatic Cloned and Normal Cow
Author(s) -
Tanaka Sachi,
Miyazawa Kohtaro,
Watanabe Kouichi,
Ohwada Shyuichi,
Aso Hisashi,
Yonai Miharu,
Saito Norio,
Yamaguchi Takahiro
Publication year - 2006
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/j.1600-0897.2005.00319.x
Subject(s) - biology , cd8 , lactation , t cell , population , flow cytometry , cloning (programming) , somatic cell , immune system , immunology , microbiology and biotechnology , andrology , medicine , genetics , gene , pregnancy , environmental health , computer science , programming language
Problem Somatic cloning technology is beneficial for genetically producing excellent animals. However, many developmental problems of somatically cloned animals have been described. Some of them may cause disorders of the immune system, resulting in the fluctuation of the proportion of white blood cells (WBC), different from that of normal animals in peripheral blood. Method of study In Holstein‐ cloned and normal cows, the fluctuation of granulocytes, monocytes, B cells and T cells, and further T cell subsets (CD4 + , CD8 + , γ δ , CD8 + γ δ and WC1 + γ δ T cell) in peripheral blood were analyzed in early lactation stage (ELS) and mid to late lactation stage (MLS) by flow cytometry using specific monoclonal antibodies for cell surface markers. Results In both ELS and MLS, there were no significant differences in the proportions of granulocytes, monocytes, B cells and T cells between cloned and normal cows. In T cell subsets, γ δ and WC1 + γ δ T cells in cloned cows were significantly less frequent than in normal cows in ELS. The decreased proportions of γ δ and WC1 + γ δ T cells recovered to the level of normal cows in MLS. Conclusions The population of granulocytes, monocytes, B cells and T cells, and T cell subsets except for γ δ and WC1 + γ δ T cells in cloned cows fluctuated in a manner similar to those of normal cows during lactation. In ELS, the proportions of γ δ and WC1 + γ δ T cells temporarily declined in cloned cows, suggesting that cloned cows may fall into an immunosuppressive state in ELS.