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The Chemokine SDF‐1/CXCL12 Contributes to T Lymphocyte Recruitment in Human Pre‐ovulatory Follicles and Coordinates with Lymphocytes to Increase Granulosa Cell Survival and Embryo Quality
Author(s) -
Kryczek Ilona,
Frydman Nelly,
Gaudin Françoise,
Krzysiek Roman,
Fanchin Renato,
Emilie Dominique,
Chouaib Salem,
Zou Weiping,
Machelon Véronique
Publication year - 2005
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/j.1600-0897.2005.00307.x
Subject(s) - cxcr4 , biology , stromal cell , chemokine , follicular phase , apoptosis , andrology , flow cytometry , microbiology and biotechnology , endocrinology , immunology , cancer research , immune system , medicine , genetics
We investigated the production and the role of the chemokine stromal cell‐derived factor‐1 (SDF‐1/CXCL12) in pre‐ovulatory follicles of women undergoing in vitro fertilization. We detected CXCL12 and its receptor CXCR4 by flow cytometry, western blotting and RT‐PCR. We tested cell migration in Transwell experiments. We measured apoptosis using ΔΨm‐sensitive fluorescent probe DiOC 6 (3) and we screened apoptosis‐related gene expression with macro‐arrays. Granulosa cells from follicular aspirates produce CXCL12 that contributes to T lymphocytes recruitment. CXCL12 reduces early apoptosis of granulosa cells. This effect is accompanied by a shift of bcl2/bax ratio, and decreased expression of p53‐targeted genes (pig7, pig8, p21, gadd45). Removal of lymphocytes disables CXCL12‐mediated anti‐apoptotic effect on granulosa cells. Anti‐apoptotic activity of CXCL12 is positively correlated to high quality of embryos. In conclusion, CXCL12 is locally produced by luteinizing granulosa cells. It specifically contributes to T lymphocytes recruitment and coordinates with local lymphocytes to increase granulosa cell survival and embryo quality.