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Regulation and Signal Transduction of Toll‐Like Receptors in Human Chorioncarcinoma Cell Lines
Author(s) -
Klaffenbach Daniela,
Rascher Wolfgang,
Röllinghoff Martin,
Dötsch Jörg,
Meißner Udo,
Schnare Markus
Publication year - 2005
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/j.1600-0897.2004.00247.x
Subject(s) - signal transduction , receptor , microbiology and biotechnology , toll , biology , transduction (biophysics) , immunology , genetics , biochemistry
Problem: Toll‐like receptors (TLRs) are expressed on placental cells. The aim of this study is to analyze signaling components activated in placenta cells after TLR ligand engagement. Methods of study: In chorioncarcimoma cell lines the regulation of TLRs was determined by real time polymerase chain reaction as well as by fluorescence‐activated cell sorter analysis. Activation of NF‐ κ B was determined in a reporter assay system and the activation of the mitogen‐activated protein kinase signaling pathways by immunoblot analysis. Results: Both lipopolysaccharide (LPS) and DNA oligonucleotides containing unmethylated CpG motifs (CpG) induced the enhanced expression of TLR2 mRNA as well as a TLR2 surface protein expression. Functionally, incubation of JAR cells with microbial stimuli such as LPS activated NF‐ κ B, as well as the phosphorylation of ERK1/2 and p38 MAP kinases and secretion of interleukin‐8. Conclusion: The functional expression of TLRs on placental cells may play an important role in the initiation of an immune response in the developing fetus.