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Cleavage of Integrin by μ ‐Calpain during Hypoxia in Human Endometrial Cells
Author(s) -
Aoyama Kazufumi,
Ozaki Yasuhiko,
Nakanishi Tamao,
Ogasawara Mayumi S.,
Ikuta Katsuo,
Aoki Koji,
Blomgren Klas,
Suzumori Kaoru
Publication year - 2004
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/j.1600-0897.2004.00236.x
Subject(s) - calpain , cytoplasm , western blot , stromal cell , hypoxia (environmental) , immunostaining , integrin , biology , microbiology and biotechnology , endometrium , chemistry , endocrinology , cancer research , cell , immunology , immunohistochemistry , biochemistry , gene , organic chemistry , oxygen , enzyme
Problem: The distribution and activation of μ ‐calpain and possible cleavage of integrin in human endometrial cells under hypoxic condition were investigated. Method of study: Human endometrial epithelial and stromal cells were subjected to hypoxia, and subsequently used for immunostaining and western blot analysis. Results: The proform of μ ‐calpain was detected in the cytoplasm of normal cells, and displayed a substantial decrease after hypoxia. Conversely, the active form of μ ‐calpain was not detected in normal cells, but was abundant after hypoxia. The cytoplasmic domain of integrin β 3 was also detected in the cytoplasm of endometrial cells. Western blot analysis confirmed that both the proform of μ ‐calpain and the integrin β 3 cytoplasmic domain decreased during hypoxia. Conclusions: μ ‐Calpain is activated in human endometrial cells during hypoxia and that subsequent cleavage of the integrin β 3 cytoplasmic domain may give some adverse effects to the function of human endometrium.