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Interleukin‐1β‐induced Expression of IL‐6 and Production of Human Chorionic Gonadotropin in Human Trophoblast Cells via Nuclear Factor‐κB Activation
Author(s) -
Tsukihara Satoru,
Harada Tasuku,
Deura Imari,
Mitsunari Masahiro,
Yoshida Souichi,
Iwabe Tomio,
Terakawa Naoki
Publication year - 2004
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/j.1600-0897.2004.00209.x
Subject(s) - trophoblast , human chorionic gonadotropin , gonadotropin , andrology , endocrinology , medicine , microbiology and biotechnology , chemistry , biology , pregnancy , placenta , fetus , hormone , genetics
Problem: The cytokine, interleukin‐6 (IL‐6), stimulates the production of human chorionic gonadotropine (hCG) in chorionic cells. The purpose of this study was to examine the role of nuclear factor‐ κ B (NF‐ κ B) during the induction of IL‐6 by IL‐1 β in human trophoblast cells. Method of study: Reverse transcription‐polymerase chain reaction was used to determine the gene expressions of IL‐1, IL‐1 receptor (IL‐1R), IL‐6R and gp130 in a human choriocarcinoma cell line, BeWo. The BeWo cells were cultured for 24 hr with IL‐1 β (0–10 ng/mL), IL‐1 β (10 ng/mL) together with IL‐1 receptor antagonist (IL‐1Ra) or NF‐ κ B inhibitor, N ‐tocyl‐ l ‐phenylalanine chloromethyl ketone (TPCK). The concentrations of IL‐6 in culture supernatants were measured by enzyme‐linked immunosorbent assay. Results: The gene expressions of IL‐1R, IL‐6R and gp130, but not IL‐1 β , were observed. The concentration of IL‐6 was enhanced by IL‐1 β in a dose‐dependent fashion. The addition of IL‐1Ra neutralized the effects of IL‐1 β on IL‐6 secretion. IL‐1 β induced expression of phosphorylated I κ B and the activation of NF‐ κ B. The addition of TPCK reduced the IL‐1 β ‐induced IL‐6 expression. Adding IL‐1 β increased β ‐hCG production in a dose‐dependent manner, and the addition of TPCK neutralized the effect of IL‐1 β . Conclusion: IL‐1 β may induce the IL‐6 expression and hCG production in human BeWo cells via NF‐ κ B.