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The Effect of Inflammatory Cytokines on Secretion of Macrophage Colony‐Stimulating Factor and Monocyte Chemoattractant Protein‐1 in Human Granulosa Cells
Author(s) -
Kawano Yasushi,
Fukuda Junichiro,
Itoh Hiroko,
Takai Noriyuki,
Nasu Kaei,
Miyakawa Isao
Publication year - 2004
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/j.1600-0897.2004.00198.x
Subject(s) - endocrinology , tumor necrosis factor alpha , medicine , monocyte , macrophage colony stimulating factor , macrophage , receptor , secretion , chemistry , cytokine , macrophage inflammatory protein , interleukin , biology , in vitro , biochemistry
Problem: In order to investigate the role of macrophage colony‐stimulating factor (M‐CSF) and monocyte chemoattractant protein ‐1 (MCP‐1) in human ovulation, we studied the regulation of M‐CSF and MCP‐1 in cultured human granulosa cells. Method of study: Immortalized granulosa cells (GC1a) were cultured in serum‐free medium, and incubated with interleukin (IL)‐1 α , IL‐1 receptor antagonist (ra) and tumor necrosis factor (TNF)‐ α . The supernatants were collected, and M‐CSF and MCP‐1 were measured by ELISA. Results: The levels of M‐CSF and MCP‐1 were increased after treatment with IL‐1 α (1 n m ) and TNF‐ α (1 n m ) in a time‐dependent manner. The levels of M‐CSF and MCP‐1 were significantly increased after treatment with IL‐1 α and TNF‐ α in a dose‐dependent manner. However, the levels of M‐CSF and MCP‐1 were significantly decreased by treatment with IL‐1 α (1 n m ) and/or increasing concentrations of IL‐1 ra. Conclusions: Our data indicated that M‐CSF and MCP‐1 were regulated by IL‐1 α and TNF‐ α . It was suggested that M‐CSF and MCP‐1 may play an important role in human preovulatory processes.