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Human Amniotic Fluid Cells are Able to Produce IL‐6 and IL‐8
Author(s) -
Mazzucchelli Iolanda,
Avanzini Maria Antonietta,
Ciardelli Laura,
Pagani Sara,
Greco Rossella,
Belloni Cesare,
Castellazzi AnnaMaria,
Marconi Massimo,
Rondini Giorgio,
Polatti Franco
Publication year - 2004
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/j.1600-0897.2004.00137.x
Subject(s) - amniotic fluid , flow cytometry , cd14 , antigen , immune system , immunology , biology , human leukocyte antigen , microbiology and biotechnology , andrology , medicine , pregnancy , fetus , genetics
Problem: In order to investigate the role of amniotic fluid cells (AFC) in the establishment of feto–maternal immune relationship, we evaluated their phenotype and capacity to produce cytokines. Methods of study: CK 7–8, human leukocyte antigen (HLA)‐I, HLA‐DR, HLA‐G, CD1d, CD34, CD45, CD14 surface antigens expression and the intracellular production of IL‐4, IL‐6, IL‐8, IL‐10, IL‐12, interferon‐ γ and tumor necrosis factor‐ α were studied in cultured AFC and in eight samples immediately after amniocentesis using flow cytometry. IL‐6 and IL‐8 were detected by ELISA in all amniotic fluids and in all culture supernatants. Moreover, IL‐6 and IL‐8 mRNA were tested in nine samples. Results: Cultured AFC express HLA‐I, HLA‐G and CK 7–8 and are able to produce IL‐6 and IL‐8, confirmed by presence of their mRNA. We quantified IL‐6 and IL‐8 levels in all amniotic fluids and in all supernatants. Conclusion: Surface antigen expression of AFC are not specific of immune cells, but AFC are able to produce cytokines and we can postulate that they may participate in mechanisms involved in normal as well as pathological pregnancy.