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Modulation of Perforin Expression in the Decidual and Peripheral Blood Cytotoxic Lymphocytes in Culture
Author(s) -
ŠTrbo N.,
Laškarin G.,
Sotošek V.,
Randić L.J.,
Podack E.R.,
Rukavina D.
Publication year - 1999
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/j.1600-0897.1999.tb00460.x
Subject(s) - cytotoxic t cell , decidua , perforin , intracellular , biology , flow cytometry , downregulation and upregulation , in vitro , chemistry , microbiology and biotechnology , andrology , endocrinology , immunology , pregnancy , placenta , medicine , fetus , biochemistry , genetics , gene
PROBLEM: Perforin (P) is a cytolytic molecule located in intracellular granules of cytotoxic lymphocytes both in the peripheral blood and decidua of pregnancy. The aim was to analyze the kinetics of P expression during in vitro culture and modulation of P expression by adherent cells, their supernatants and mitogen (PHA) stimulation. METHOD OF STUDY: P (intracellular antigen) was detected by flow cytometry in the suspension of first trimester pregnancy peripheral blood lymphocytes (PBL) and decidual lymphocytes (DL). RESULTS: A decrease of the percentage of P + cells was obtained after 1 hr incubation and was prevented by addition of 30% of decidual adherent cells (DAC) or their supernatants. Upregulation of P expression was obtained when, in addition to adherent cells, DL and PBL were stimulated by PHA. DAC present in the culture in physiological concentrations prevent downregulation of P expression. CONCLUSION: DAC located in the vicinity of decidual cytotoxic lymphocytes, owing to their unique ability to produce a wide range of substances on demand, contribute to the high level of P expression in the decidua of pregnancy.