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Mouse Granulated Metrial Gland Cell Cytotoxicity of Wehi 164 Cells: Is There a Role for Interleukin‐3 and Tumour Necrosis Factor‐α?
Author(s) -
Stewart I.J.,
Peel S.
Publication year - 1999
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/j.1600-0897.1999.tb00457.x
Subject(s) - cytotoxic t cell , tumor necrosis factor alpha , cytotoxicity , endocrinology , biology , medicine , interleukin , chemistry , cytokine , in vitro , immunology , biochemistry
PROBLEM: Mouse granulated metrial gland (GMG) cells are maternal, cytotoxic cells found in the uterine wall in pregnancy. In vivo , they are cytotoxic to layer 1 labyrinthine placental cells. In an in vitro system, granulated metrial gland cells are highly cytotoxic to Wehi 164 fibrosarcoma cells. In this study the role of interleukin (IL)‐3 and tumour necrosis factor (TNF)‐α in granulated metrial gland cell cytotoxic activity was investigated. METHOD OF STUDY: Chromium‐release cytotoxicity assays using mouse metrial gland effector cells and Wehi 164 target cells with the addition of IL‐3 or an antibody to TNF‐α. RESULTS: An antibody to TNF‐α reduced the level of cytotoxic activity. IL‐3 had no effect on the level of cytotoxicity. CONCLUSION: Mouse granulated metrial gland cells kill using a TNF‐α mediated mechanism which is independent of IL‐3 stimulation.