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The Expression of Granulocyte Macrophage‐Colony Stimulating Factor (GM‐CSF) and Receptors in Human Endometrium *
Author(s) -
Zhao Yong,
Chegini Nasser
Publication year - 1999
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/j.1600-0897.1999.tb00106.x
Subject(s) - endometrium , autocrine signalling , biology , paracrine signalling , medicine , endocrinology , receptor , granulocyte macrophage colony stimulating factor , stromal cell , macrophage colony stimulating factor , in situ hybridization , messenger rna , microbiology and biotechnology , cytokine , macrophage , immunology , cancer research , gene , biochemistry , in vitro
Zhao Y, Chegini N. The expression of granulocyte macrophage‐colony stimulating factor (GM‐CSF) and receptors in human endometrium. AJRI 1999; 42:303–311 © Munksgaard, Copenhagen PROBLEM: To determine the temporal and spatial expression of granulocyte macrophage‐colony stimulating factor (GM‐CSF) and GM‐CSF α and β receptor mRNA and protein in human endometrium. METHOD OF STUDY: The endometrial expression of GM‐CSF and GM‐CSF receptor mRNA and protein was determined using competitive quantitative reverse transcription polymerase chain reaction (Q‐RT‐PCR), in situ hybridization, and immunohistochemistry. RESULTS: Endometrium expresses GM‐CSF and GM‐CSF α receptor mRNA with maximal expression occurring during the mid‐secretory phase (21.1 ±4.2 and 32.2 ± 7.7 × 10 6 mRNA copies/μg total RNA) compared to the proliferative phase (1.46 ± 0.4 and 7.5 ± 0.5 × 10 6 copies) of the menstrual cycle, with a significant reduction (0.67 ± 0.1 and 1.7 ± 0.2 × 10 6 mRNA copies) during the post‐menopausal period ( P < 0.05). The endometrium expresses a significantly lower level of GM‐CSF β receptor mRNA (≈ 0.01 × 10 5 mRNA copies). Endometrial luminal and glandular epithelial cells are the primary site of GM‐CSF mRNA and protein expression, while arteriole endothelial, stromal, and inflammatory cells are the primary site of GM‐CSF α receptor protein. GM‐CSF β receptor protein has a similar cellular distribution as GM‐CSF. CONCLUSION: Temporal and spatial expression of GM‐CSF and GM‐CSF receptors in human endometrium during the menstrual cycle suggests that epithelial‐derived GM‐CSF in an autocrine/paracrine manner may influence various endometrial biological activities, local inflammatory response, and macrophage survival.