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Interleukin‐6 Levels in Co‐culture of Human In Vitro Fertilization Embryos with Vero Cells Are Not Predictive of Future Successful Development
Author(s) -
LapréeDelage Geneviève,
Volante Monique,
Frydman René,
Chaouat Gérard
Publication year - 1999
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/j.1600-0897.1999.tb00090.x
Subject(s) - human fertilization , in vitro , embryo , in vitro fertilisation , biology , vero cell , andrology , embryogenesis , cryopreservation , microbiology and biotechnology , immunology , medicine , anatomy , genetics
PROBLEM: In an attempt to predict successful embryo transfer and implantation, we measured interleukin (IL)‐6 levels in culture supernatants of co‐cultured preimplantation human embryos. We tested whether all in vitro —fertilized human embryos in co‐cultures do secrete IL‐6, and whether there was any difference in such production between embryos that successfully reached the blastocyst stage and blocked embryos. We also addressed the question of IL‐6 secretion by co‐culture support cells, namely Vero cells themselves. METHOD OF STUDY: Each fertilized oocyte was cultured individually and transferred in culture wells supplemented with a feeder layer of Vero cells at day 2. In vitro IL‐6 production was measured by bioassay of the culture media. RESULTS: Because Vero cells themselves secrete IL‐6, it became impossible, in co‐culture, to quantify production of IL‐6 by the sole embryos. On the other hand, the co‐culture technique has shown us that embryos are likely to consume IL‐6. There was no difference between blastocysts and blocked embryos. CONCLUSIONS: IL‐6 levels in human embryo co‐cultures do not correlate with future successful embryo transfer.