Premium
Analysis of Human Leukocyte Antigen‐G Polymorphism Including Intron 4 in Japanese Couples with Habitual Abortion
Author(s) -
Yamashita Takahiro,
Fujii Tomoyuki,
Tokunaga Katsushi,
Tadokoro Kenji,
Hamai Yoko,
Miki Akinori,
Kozuma Shiro,
Juji Takeo,
Taketani Yuji
Publication year - 1999
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/j.1600-0897.1999.tb00089.x
Subject(s) - intron , single strand conformation polymorphism , human leukocyte antigen , exon , genetics , biology , allele , gene , polymerase chain reaction , abortion , polymorphism (computer science) , microbiology and biotechnology , antigen , pregnancy
PROBLEM: The purpose of this study was to clarify whether there is a difference between the allele frequency of human leukocyte antigen (HLA)‐G in healthy Japanese people and that of Japanese couples with habitual abortion. METHOD OF STUDY: Exons 2, 3, 4, and intron 4 of the HLA‐G gene were analyzed in 20 couples with habitual abortion, using polymerase chain reaction (PCR)‐single‐strand conformation polymorphism (SSCP) analysis. Intron 4 of the HLA‐G gene was also analyzed in 54 healthy individuals. The nucleotide sequence of the PCR product of intron 4 was further determined by direct sequencing. RESULTS: Two kinds of nucleotide sequence were identified in intron 4 of the HLA‐G gene, one of which was identical to that of HLA‐G*01011, and the other was identical to that of HLA‐G*01012, G*01013, and G*0104. The frequency of each allele in affected women and their husbands did not significantly differ from that of healthy individuals, and no mutation was found in any affected couple. CONCLUSION: HLA‐G allelic abnormality seemed to have little, if any, implication in the pathogenesis of habitual abortion.