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The Expression of Human Leukocyte Antigen‐G on Trophoblasts Abolishes the Growth‐Suppressing Effect of Interleukin‐2 towards Them
Author(s) -
Hamai Yoko,
Fujii Tomoyuki,
Yamashita Takahiro,
Miki Akinori,
Hyodo Hironobu,
Kozuma Shiro,
Geraghty Daniel E.,
Taketani Yuji
Publication year - 1999
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/j.1600-0897.1999.tb00088.x
Subject(s) - hla g , transfection , trophoblast , biology , cytokine , human leukocyte antigen , preeclampsia , immunology , cytotoxic t cell , cell culture , receptor , cell growth , antigen , endocrinology , fetus , pregnancy , placenta , in vitro , biochemistry , genetics
PROBLEM: We have shown the attenuated human leukocyte antigen (HLA)‐G expression on trophoblasts and an aberrant expression of interleukin (IL)‐2, a cytotoxic cytokine, in decidual tissue in preeclampsia, where deteriorated trophoblastic invasion into decidual layers may constitute a crucial pathogenesis. We hypothesized that the absence of HLA‐G might make trophoblasts susceptible to compromise by IL‐2. METHOD OF STUDY: We analyzed the growth of HLA‐G‐negative and ‐positive cell lines, all of which possessed IL‐2 receptors, in the culture with or without IL‐2 supplementation. RESULTS: The proliferation of HLA‐G‐positive trophoblastic cell lines (BeWo and JEG‐3) was not influenced by the addition of IL‐2, whereas a HLA‐G‐negative trophoblastic cell line (JAR) exhibited significantly decreased proliferation when cultured with IL‐2. Interestingly, the transfection of JAR cells with HLA‐G completely eliminates the growth‐inhibitory effect of IL‐2. CONCLUSION: The expression of HLA‐G may commit trophoblasts to evade cell damage by IL‐2, which may be relevant to maternal tolerance of the fetus during pregnancy and its derangement as exemplified by preeclampsia.