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Peripheral Blood Mononuclear Cells from Women with Recurrent Abortion Exhibit an Aberrant Reaction to Release Cytokines upon the Direct Contact of Human Leukocyte Antigen‐G‐Expressing Cells
Author(s) -
HAMAI YOKO,
FUJII TOMOYUKI,
YAMASHITA TAKAHIRO,
MIKI AKINORI,
KOZUMA SHIRO,
GERAGHTY DANIEL E.,
TAKETANI YUJI
Publication year - 1998
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/j.1600-0897.1998.tb00426.x
Subject(s) - peripheral blood mononuclear cell , human leukocyte antigen , hla g , immunology , abortion , medicine , tumor necrosis factor alpha , antigen , pathogenesis , interleukin , cytokine , pregnancy , biology , in vitro , biochemistry , genetics
PROBLEM: In search for pathogenesis of recurrent abortion, we examined whether lymphocytes/macrophages from women with recurrent abortion exhibited an aberrant ability to release cytokines upon the direct contact of human leukocyte antigen (HLA)‐G. METHOD OF STUDY: The amounts of cytokines released from peripheral blood mononuclear cells (PBMCs) from women with recurrent abortion were compared with those from normal multiparous women or normal nulligravidous women when cocultured with or without HLA‐G‐expressing target cells. RESULTS: When cocultured with HLA‐G‐expressing target cells, the amount of interleukin‐1β released from PBMCs was increased in recurrent aborters whereas it decreased in both normal multiparous and nulligravidous women. The amount of interleukin‐3 released from PBMCs did not differ with or without HLA‐G‐expressing cells in recurrent aborters, whereas it increased in the presence of HLA‐G‐expressing cells in normal controls. The amount of tumor necrosis factor‐α released from PBMCs was decreased in the presence of HLA‐G‐expressing cells in both recurrent aborters and normal controls. CONCLUSION: The aberrant reaction of maternal lymphocytes/macrophages in releasing cytokines upon the contact of HLA‐G expressed on trophoblasts may impact negatively on trophoblastic growth, which may be pathogenic in recurrent abortion.