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Assessment of Progesterone‐Induced Acrosome Reaction by Biotinylated Monoclonal Antibody Probes
Author(s) -
Lee ChiYu Gregory,
Khorasani Afsaneh Motamed,
Dorjee Sonam
Publication year - 1998
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/j.1600-0897.1998.tb00350.x
Subject(s) - biotinylation , monoclonal antibody , acrosome reaction , monoclonal , chemistry , antibody , biology , microbiology and biotechnology , andrology , immunology , medicine , sperm
PROBLEM: To develop an immunohistochemical assay for determination of acrosome‐reacted human sperm and to study the effects of progesterone and cholesterol treatment on human sperm acrosome reaction. METHOD OF STUDY: Three distinct anti‐sperm monoclonal antibodies were biotinylated and used as probes for assessment of acrosome reaction in a 30‐min immunohistochemical assay. Progesterone and/or cholesterol were added to sperm preparation to influence the acrosome reaction in different experimental conditions. RESULTS: Percentages of acrosome‐intact sperm decreased significantly during the 18‐hr incubation. Acrosome reaction could be induced by progesterone as early as 2 hr after sperm incubation in human tubal fluid. The degree of progesterone‐induced acrosome reaction was time dependent and the optimal effect was reached by adding 10 μg/ml progesterone for 30 min incubation. Progesterone‐induced acrosome reaction was shown to be hormone‐concentration dependent with 50% stimulation at 1 μg/ml. Cholesterol (1 μg/ml) was found to inhibit progesterone‐induced acrosome reaction either by co‐incubation with human sperm during capacitation, or by simultaneous incubation with progesterone during acrosome reaction induction. CONCLUSIONS: Assessment of human sperm acrosomal status by avidin‐biotin immunohistochemical assay can be a routine in clinical laboratories for male infertility services. Cholesterol can inhibit progesterone‐induced acrosome reaction, possibly by its modifications of sperm plasma membrane and/or interference of progesterone binding to its surface receptors.