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Comparative Studies on the Effects of Interleukin‐4 and Interleukin‐13 on Cytokine and Prostaglandin E 2 Production by Amnion‐derived WISH Cells
Author(s) -
Keelan Jeffrey A.,
Sato Timothy A.,
Mitchell Murray D.
Publication year - 1998
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/j.1600-0897.1998.tb00062.x
Subject(s) - cytokine , endocrinology , medicine , prostaglandin e , interleukin , amnion , biology , interleukin 2 , interleukin 22 , prostaglandin e2 , chemistry , pregnancy , fetus , genetics
PROBLEM: In hematopoietic cells, interleukin (IL)‐13 shares many actions with IL‐4. The effects of IL‐13 in gestational tissues have yet to be reported, however. We compared the effects of IL‐4 and IL‐13 on the production of cytokines and prostaglandin E 2 (PGE 2 ) in epithelial amnion‐derived WISH cells. METHOD OF STUDY: WISH cells were treated with IL‐4 or IL‐13 (0.08–10 ng/ml) with/without cotreatment with IL‐1β (0.2 ng/ml), tumor necrosis factor‐α (10 ng/ml) or epidermal growth factor (5 ng/ml). The production of IL‐6, IL‐8, and PGE 2 was measured by immunoassay after 16 hr. RESULTS: Both IL‐4 and IL‐13 inhibited PGE 2 production with indistinguishable concentration‐response curves, under basal or stimulated conditions. The maximal inhibition of IL‐1β‐stimulated PGE 2 production (to 28% ± 10% of control) was seen at 10 ng/ml of IL‐4 or IL‐13. Basal IL‐6 production was stimulated approximately twofold by IL‐4 and IL‐13, whereas IL‐4 and IL‐13 both inhibited cytokine‐stimulated (but not basal) IL‐8 production by approximately 50%. In the presence of 1 ng/ml of IL‐4, IL‐13 was unable to further inhibit PGE 2 production. CONCLUSIONS: The inhibition of PGE 2 and IL‐8 production by IL‐4 in WISH cells is mimicked by IL‐13. Both cytokines, probably through binding to a common receptor complex, may share a role in suppressing inflammatory reactions within intrauterine tissues.