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The Effect of Interleukin‐1β and Interleukin‐4 on the Expression of Prostaglandin Receptors EP1 and EP3 in Amnion WISH Cells
Author(s) -
Spaziani Eric P.,
Tsibris John C. M.,
Hunt Lois T.,
Benoit Raymond R.,
O'Brien William F.
Publication year - 1997
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/j.1600-0897.1997.tb00515.x
Subject(s) - amnion , receptor , microbiology and biotechnology , interleukin 22 , interleukin , interleukin 1β , interleukin 2 , medicine , prostaglandin , prostaglandin e2 , biology , endocrinology , cytokine , chemistry , immunology , pregnancy , genetics , fetus
PROBLEM: Although prostaglandin E 2 (PGE 2 ) is believed to modulate biochemical and immunological events leading to parturition, the role of prostaglandin E receptors during labor has not been investigated. METHOD OF STUDY: Amnion WISH cells were incubated in media containing increasing concentrations of either interleukin‐1β (IL‐1β) or IL‐4. Increased EP1 and EP3 protein expression was determined by Western blot analysis with peptide‐specific antibodies. Concomitant measurements of culture media PGE 2 were made by an enzyme immunoassay. RESULTS: Incubation of WISH cells with IL‐1β or IL‐4 caused a two‐to three‐fold increase in EP1 protein levels. IL‐1β and IL‐4 also caused six‐and two‐fold increases, respectively, in culture fluid PGE 2 concentrations. IL‐1β or IL‐4 had no effect on EP3 protein levels. CONCLUSIONS: Based on these results, it is proposed that IL‐1β and IL‐4 may be involved in the initiation and promotion of labor by inducing EP1 levels and PGE 2 production in amnion.