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Immune Responses of Chemically Modified Homologous LDH‐C4 and Their Effect on Fertility Regulation in Mice
Author(s) -
Gupta G.S.,
Syal N.
Publication year - 1997
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/j.1600-0897.1997.tb00215.x
Subject(s) - immune system , splenocyte , andrology , antibody , adjuvant , sperm , antigen , lactate dehydrogenase , immunology , polyclonal antibodies , biology , chemistry , biochemistry , medicine , enzyme
PROBLEM: Lactate dehydrogenase‐C 4 (LDH‐C 4 ) is an iso‐, allo‐ and auto‐antigen of mammalian sperm. In spite of being cell specific, LDH‐C 4 does not induce infertility in females of homologous species after immunization. The present study examines the application of homologous LDH‐C 4 after chemical modifications in the immunological infertility of female mice. METHODS: LDH‐C 4 from testes of LACA mice was chemically modified by interacting it with gossypol (gossy‐LDH‐C 4 ) and glucosylation with lactose (Glu‐LDH‐C 4 ) in vitro and evaluated for immune responses and induced immunological infertility in allogeneic Balb/c mice after inoculation through intrarectal route using Al(OH) 3 as adjuvant. RESULTS: Native LDH‐C 4 , which elicited high antibody response after immunization with a primary (50 μ g ) and three secondary doses (30 μg each) at an interval of 15 days each, did not reduce fertility significantly in mice. In contrast, study provides evidence that chemically modified LDH‐C 4 induces high infertility, since 85–100% of mice failed to conceive in two independent sets of experiments. Mice inoculated with modified LDH‐C 4 were associated with 2–3‐fold anti‐LDH‐C 4 antibody titre compared to antibody response elicited by native LDH‐C 4 . Splenocytes from immunized non‐pregnant mice were evaluated for polyclonal activation using Con A as mitogen. It was found that splenocytes primed with native LDH‐C 4 were significantly more stimulated than the non‐immune control cultures. However cells primed with gossy‐LDH‐C 4 were non‐reactive to Con A and cells primed with glu‐LDH‐C 4 were suppressed for ConA proliferation. CONCLUSIONS: It is concluded that LDH‐C 4 ‐gossypol adduct offers a potential application in the induction of infertility of homologous species in marked contrast to native LDH‐C 4 . Application of Al(OH)3 as adjuvant in the induction of immune response through intra‐rectal route has been suggested.