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Globulin‐Enriched Protein Supplements Shorten the Pre‐Compaction Mitotic Interval and Promote Hatching of Murine Embryos
Author(s) -
Schneider E. Gayle,
Hayslip C. Calvin
Publication year - 1996
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/j.1600-0897.1996.tb00147.x
Subject(s) - hatching , embryo , andrology , mitosis , biology , globulin , microbiology and biotechnology , embryogenesis , zoology , immunology , medicine
PROBLEM: To determine whether Synthetic Serum Substitute (SSS), which contains human globulins in addition to Human Serum Albumin (HSA), is superior to HSA alone as a protein supplement for embryo culture. METHOD: Development of mouse zygotes to eight‐cell/compacting morulae and to hatching/hatched blastocysts was assessed in Human Tubal Fluid (HTF) medium containing either SSS or HSA. RESULTS: Although there was no difference in the overall blastocyst rate at 120 h in HTF+SSS versus HTF+HSA, significantly more embryos at 54 h were at the eight‐cell/compacting morula stage in HTF+SSS. At 120 h, there were more hatching/hatched blastocysts in HTF+SSS, and hatching correlated with SSS concentration. Addition of isolated globulins to HSA significantly stimulated the number of hatching/hatched blastocysts. Hatching could be “rescued” by transfer of embryos grown in HTF+HSA to globulin‐containing media and prevented by removal of globulins as late as the compacted morula stage (54 h). CONCLUSIONS: SSS is superior to HSA alone for embryo culture. The stimulatory effects on mitosis and hatching may be mediated directly by globulins or by other components in the globulin‐enriched fraction.