Premium
Expression of Phosphatidylserine‐Dependent Antigens on the Surface of Differentiating BeWo Human Choriocarcinoma Cells
Author(s) -
Rote Neal S.,
Chang Joshua,
Katsuragawa Hiroshi,
Ng Ah Kau,
Lyden Timothy W.,
Mori Takahide
Publication year - 1995
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/j.1600-0897.1995.tb01147.x
Subject(s) - antigen , monoclonal antibody , phosphatidylserine , cytotrophoblast , microbiology and biotechnology , biology , choriocarcinoma , chemistry , forskolin , antibody , syncytiotrophoblast , cell culture , immunology , placenta , biochemistry , phospholipid , pregnancy , fetus , genetics , membrane
PROBLEM: Antiphospholipid antibodies (aPLs) are associated with pregnancy loss, pregnancy‐induced hypertension, and intrauterine growth retardation. We have previously reported that phosphatidylserine (PS)‐dependent antigens are expressed in formalin‐fixed cells concurrent with differentiation in a choriocarcinoma model (BeWo) of cytotrophoblast. That study, however, could not differentiate between cytoplasmic or surface antigen expression. METHOD: Three monoclonal aPLs that differentiate between PS‐ and cardiolipin (CL)‐dependent antigens were reacted with BeWo, with or without forskolin activation, before fixation, and antibody binding was evaluated by immunoperoxidase techniques. RESULTS: Activation with forskolin induced a PS‐dependent antigenic determinant on the surface on BeWo cells. CL‐reactive monoclonal antibodies did not react with the cell surface, whether forskolin treated or not. CONCLUSION: These observations demonstrate that a PS‐dependent antigen is expressed on the surface of a model of differentiating cytotrophoblastic cells and should be accessible in vivo to circulating aPLs.