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Regulation of Classical HLA Class I Genes in Human Choriocarcinoma Cells by Nuclear Proteins Binding to MHC Class I Regulatory Elements
Author(s) -
WAKIMOTO AKINORI,
OHASHI KAZUTOMO,
KOYAMA MASAYASU,
KATO MUNEHIRO,
TSUTSUI TATEKI,
SAJI FUMITAKA,
TANIZAWA OSAMU
Publication year - 1995
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/j.1600-0897.1995.tb00959.x
Subject(s) - human leukocyte antigen , choriocarcinoma , electrophoretic mobility shift assay , mhc class i , biology , microbiology and biotechnology , gene , nuclear protein , cd74 , dna binding protein , genetics , transcription factor , major histocompatibility complex , antigen
PROBLEM : The regulation of classical HLA class I genes in choriocarcinoma have been reported. METHODS : We determined whether four choriocarcinoma cell lines expressed classical HLA class I or HLA‐G by a reverse transcription‐polymerase chain reaction (RT‐PCR) and studied the regulatory mechanism of classical class I using a gel mobility shift assay. RESULTS : NUC1 and SCH expressed classical class I but not HLA‐G. GCH1 and Jar did neither. Nuclear protein binding to the class I regulatory element (CRE) was detected in NUC1 and SCH. Interferon‐γ augmented both classical class I expression and the DNA‐protein complex in NUC1. The DNA‐protein complex was not observed in GCH1, and Jar showed a CRE‐binding protein with different electrophoretic mobility and binding affinity from that of SCH and NUC1. CONCLUSION : The CRE is one of the regulatory elements of classical HLA class I genes in choriocarcinoma cells.