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Hematopoietic Stem Cell Antigen‐1 (Sca‐1) Expression in Different Lymphoid Tissues of Female Mice Treated With GnRH Agonist
Author(s) -
RAO L. V.,
CLEVELAND R.P.,
KIMMEL R.J.,
ATAYA K.M.
Publication year - 1995
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/j.1600-0897.1995.tb00950.x
Subject(s) - spleen , stem cell , endocrinology , haematopoiesis , bone marrow , agonist , medicine , biology , flow cytometry , immunology , receptor , microbiology and biotechnology
PROBLEM : Our earlier studies have demonstrated a general suppression of leukocyte maturation upon GnRH agonist treatment in mice and suggested a potential effect at an early stem cell stage of leukocyte development. METHOD : Three‐week old Balb/c and C57BL/6 female mice received 50 μlg injections of Lupron depot or placebo. Sequential changes in Sca‐1 + cells in the bone marrow, thymus, blood and spleen were studied by flow cytometry. RESULTS : In bone marrow, the absolute numbers of Sca‐1 + cells were significantly decreased at 2 weeks in C57BL/6 mice whereas a decreasing trend was noted in Balb/c mice following agonist administration. Concomitantly, thymocytes expressing Sca‐1 + cells were significantly increased at 2 weeks in C57BL/6 mice, but were significantly decreased in Balb/c mice. Significant decreases in Sca‐1 + cells were also observed in spleen and blood in Balb/c mice whereas no significant differences were observed in C57BL/6 mice. CONCLUSIONS : These data suggest GnRH agonists affect hematopoietic stem cell development in mice. The effects observed vary with different genetic backgrounds. In Balb/c mice these effects are more pronounced, and appear to result in the inhibition of stem cell maturation. In contrast, GnRH agonist enhances stem cell maturation in C57BL/6 mice.