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Chymotrypsin‐Galactose Treatment of Sperm With Antisperm Antibodies Results in Improved Pregnancy Rates Following in Vitro Fertilization
Author(s) -
KATSOFF DIANE,
CHECK JEROME H.,
BOLLENDORF ANIELA,
BENFER KELLY
Publication year - 1995
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/j.1600-0897.1995.tb00878.x
Subject(s) - sperm , human fertilization , in vitro fertilisation , insemination , andrology , pregnancy , antibody , semen , pregnancy rate , fertility , medicine , biology , gynecology , immunology , population , anatomy , environmental health , genetics
PROBLEM : To determine if chymotrypsin‐galactose (CG) treatment of sperm bound with antisperm antibodies (ASA) improves pregnancy rates (PRs) following in vitro fertilization (IVF). METHOD : Patients with >50% ASA who failed to conceive despite six intrauterine insemination (IUI) cycles were included. Initially the sperm treatments were randomized with CG vs culture medium; subsequently only CG treatment was used. RESULTS : There was a significantly lower fertilization rate in those patients inseminated with sperm incubated in culture medium vs CG (27% vs 47%, P <.05 t‐test). Similarly, a higher percentage of patients receiving culture medium treatment of sperm had failed fertilization (45%) compared to CG (11%). Though the clinical PRs were higher with CG (21%) than medium (9.5%), there was no statistical difference. CONCLUSIONS : Though the percentage of sperm bound with antibodies are not reduced, we hypothesize that the CG treatment improves fertility by possibly mitigating the antagonistic action of these antibodies.