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Immunization of Mice Against a Synthetic N‐Terminal Extracellular Domain Gonadotropin‐Releasing Hormone Receptor Peptide: Evidence for a Direct Uterine Effect
Author(s) -
Asirvatham Angela L.,
Johnson Gregory A.,
Belden E. Lee,
Kirk Edward A. Van,
Moss Gary E.,
Murdoch William J.
Publication year - 1994
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/j.1600-0897.1994.tb01099.x
Subject(s) - immunization , receptor , uterus , antibody , active immunization , endocrinology , medicine , antigen , biology , gonadotropin releasing hormone , hormone , extracellular , hormone receptor , peptide , endometrium , immunology , luteinizing hormone , microbiology and biotechnology , biochemistry , cancer , breast cancer
PROBLEM: Immature male and female mice were immunized with a synthetic peptide corresponding to amino acids 5–17 (ASLEQDPNHCSAI) of the mouse hypophyseal gonadotropin‐releasing hormone (GnRH) receptor. METHOD: Effect of immunization (postpuberal) was restricted to the uterus. Pituitary‐go‐nadal functions were not altered. RESULTS: The endometrial lining of immunized females was thin and lacked glandular development. These observations were corroborated in actively immunized and passively immunized adult females. CONCLUSIONS: Apparently endometrial cells express a unique surface antigen, though reactive with antipeptide antibodies, that differs from the prototype pituitary GnRH receptor. Antibodies that selectively inhibit endometrial maturation might be used to treat proliferative diseases of the uterus.