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Interleukin‐1 Receptor Antagonist (IL‐1ra) Production by Human Amnion, Chorion, and Decidua
Author(s) -
Fidel P.L.,
Romero R.,
Ramirez M.,
Cutright J.,
Edwin S.S.,
Lamarche S.,
Cotton D.B.,
Mitchell M.D.
Publication year - 1994
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/j.1600-0897.1994.tb00872.x
Subject(s) - decidua , amnion , interleukin 1 receptor antagonist , receptor antagonist , antagonist , receptor , endocrinology , andrology , medicine , microbiology and biotechnology , biology , chemistry , pregnancy , fetus , placenta , genetics
PROBLEM: This study was conducted to determine whether (1) conditioned media from unstimulated primary cultures of human amnion, chorion, or decidua contain detectable concentrations of IL‐lra in vitro, and (2) bacterial endotoxin (LPS), tumor necrosis factor‐alpha (TNF‐α), or IL‐1‐beta (IL‐1β) stimulate amnion, chorion, or decidua to produce increased amounts of IL‐1ra. METHOD: Placentae were obtained from women at term with intact membranes before the onset of labor. Amnion, chorion, and decidual cells were isolated by standard procedures and grown to confluence. Cells were then cultured in quadruplicate for 16 h in tissue culture medium supplemented with 10% fetal calf serum or, additionally, with various concentrations of Eschrichia coli LPS, TNF‐α, or IL‐1β. Culture supernatants were collected, and concentrations of IL‐1ra were quantitated by a sensitive and specific enzyme‐linked immunosorbent assay for IL‐lra. RESULTS: Results showed that primary cultures of amnion and chorion from 4 of 9 and decidua from 10 of 12 placentae had detectable rates of production of IL‐lra (ranges: 0.08‐6.5, 0.42‐12.1, and 1.55–96.5 pg IL‐1ra/μg protein/16 h, respectively). In addition, LPS (10‐1,000 ng/ml) and IL‐1β (0.1–10 ng/ml), but not TNF‐α (0.01–100 ng/ml), stimulated decidual cells to release/secrete increased amounts of IL‐lra compared with media alone (range: 2.5–400 pg IL‐lra/μg protein/16 h, P < 0.0001). In contrast, neither LPS, TNF‐α, or IL‐1β could stimulate amnion or chorion to release/secrete IL‐1ra. CONCLUSIONS: These results indicate (1) that amnion, chorion, and predominantly decidua, can release or secrete IL‐1ra in vitro, and (2) that LPS and IL‐1β can stimulate decidual cells to produce increased amounts of IL‐lra.