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Cyclosporin A Attenuates Increased Prostaglandin and Thromboxane Production in Response to Various Stimuli in Human Decidua
Author(s) -
EDWIN S.S.,
BRANCH D.W.,
SCOTT J.R.,
SILVER R.M.,
MITCHELL M.D.
Publication year - 1993
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/j.1600-0897.1993.tb00616.x
Subject(s) - ionomycin , thromboxane b2 , decidua , endocrinology , prostaglandin e2 , medicine , phorbol , decidual cells , radioimmunoassay , thromboxane , prostanoid , prostaglandin e , prostaglandin , chemistry , biology , stimulation , fetus , pregnancy , placenta , platelet , biochemistry , protein kinase c , signal transduction , genetics
PROBLEM: This study was undertaken to evaluate the effects of cyclosporin A on prostanoid (prostaglandin and thromboxane) production by human decidua. METHOD: Decidual cells were isolated from term placentae obtained at elective cesarean section before the onset of labor. Cells were grown to confluence and then incubated for 16 h with cyclosporin A (0.1–100 ng/ml) in the presence and absence of interleukin 1β (IL‐1β, 10 ng/ml), phorbol 12‐myristate 13‐acetate (PMA, 10 −7 M) and ionomycin (0.5 μm). Prostaglandin E 2 (PGE 2 ) and thromboxane B 2 (TXB 2 ) were measured by radioimmunoassay, and cellular protein was determined. RESULTS: IL‐1β, PMA, and ionomycin all stimulated decidual PGE 2 and TXB 2 production as expected. However, these stimulatory actions were attenuated by 20% when cells were coincubated with cyclosporin A (100 ng/ml). All concentrations of cyclosporin A tested were within the therapeutic range. CONCLUSIONS: Our results indicate that cyclosporin A does not stimulate decidual prostanoid production and is probably unrelated to preterm labor and delivery in allograft recipients.