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Absence of Immunoreactive Cytokines in Supernatants of Individual Preimplantation Human Embryos
Author(s) -
SEIFER DAVID B.,
ROMERO ROBERTO,
BERLINSKY DAVID,
HANING RAY V.
Publication year - 1993
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/j.1600-0897.1993.tb00610.x
Subject(s) - embryo , cytokine , andrology , biology , immunology , microbiology and biotechnology , medicine
PROBLEM: The purpose of this study was to determine if it was possible to use commercially available enzyme‐linked immunosorbent assay (ELISA) kits to quantitate specific cytokine production from individual embryos. METHODS: We examined the supernatants of individual two to six cell preimplantation human embryos using commerically available kits for IL‐1 alpha, IL‐1 beta, IL‐6, and LIF. RESULTS: Cytokines were undetectable in all media samples analyzed. CONCLUSION: While it is possible that individual preimplantation embryos may produce cytokines, we believe these concentrations are below the detectable limits of the commercial assays used in this study.