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Platelet‐Activating Factor Production From In Vitro and In Vivo Fertilized Murine Embryos Is Similar
Author(s) -
RIPPS B.A.,
ZHU Y.P.,
BURWINKEL T.H.,
KIM H.N.,
BUSTER J.E.,
MINHAS B.S.
Publication year - 1993
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/j.1600-0897.1993.tb00609.x
Subject(s) - embryo , in vivo , andrology , human fertilization , embryogenesis , biology , in vitro , platelet activating factor , blastocyst , in vitro fertilisation , embryo culture , immunology , microbiology and biotechnology , anatomy , genetics , medicine
PROBLEM: This study measured platelet‐activating factor (PAF) production of in vitro and in vivo fertilized murine embryos to ascertain whether site of fertilization impacted subsequent embryonic PAF production. METHOD: Ooocytes and embryo were collected from stimulated B 6 D 2 F 1 mice. PAF production throughout the preimplantation phase from the two‐celled stage through expanded blastocysts was measured and compared among normally developing embryos with the only difference being site of fertilization. RESULTS: A striking increase in PAF production was noted for both in vivo and in vitro fertilized embryos over the four days of culture. Significantly higher (P<.001) levels of PAF production were noted with development from the two‐celled stage (47.22 ± 4.13; 44.30 ± 2.43) to expanded blastocysts (254.31 ± 24.01;255.11 ± 5.35 ng/embryo/24h)forbothin vitro and in vivo fertilized embryos, respectively. There was no significant difference ( P >.05) in PAF production between the two groups of embryos. CONCLUSIONS: Embryonic PAF production in the mouse increases in a stage‐specific manner, and specifically the site of fertilization in vitro versus in vivo does not affect PAF production in normally developing embryos.