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Human Embryo Associated Immunosuppressor Factor and Serum Estradiol Synergism in Ovarian Follicle Development
Author(s) -
BOSE RATNA,
KREDENTSER J. V.,
MCCOSHEN JOHN A.
Publication year - 1993
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/j.1600-0897.1993.tb00607.x
Subject(s) - embryo , andrology , ovarian follicle , follicle , biology , anti müllerian hormone , ovarian tissue , ovary , medicine , endocrinology , hormone , microbiology and biotechnology
PROBLEM: The purpose of this study was to test whether the level of serum immunosuppressor factor (EASF) correlates with ovarian follicle development and with serum hormones during the ovarian cycle. METHOD: One hundred seventy‐three serum samples were collected starting at the time of ovulation and 1 wk after insemination from 29 women attempting pregnancy by ovarian hyperstimulation and intrauterine insemination. After ovarian stimulation, the patients were given human chorionic gonadotropin (hCG) 38 h before insemination. Number and size of follicles were measured by ultrasound. Serum samples were tested for hCG. estradiol (E 2 ). luteinizing hormone, follicle‐stimulating hormone (FSH), and progesterone levels. The level of EASF in patients' sera was assayed using a two‐site sandwich enzyme‐linked immunosorbent assay system. The levels of different hormones and EASF were then correlated among themselves and with the number of ovarian follicles. RESULTS: Eleven out of 29 patients had detectable levels of serum EASF‐Patients positive for EASF had a significantly higher number ovarian follicles ≥ 13 mm and E 2 levels after hCG treatment as compared to patients with no detectable EASF. The levels of EASF correlated with number of follicles (P = .001). blood E 2 ( P < .02) and FSH levels ( P < .02). Number of follicles ≥ 13 mm correlated with E 2 ( P = .01) and FSH levels ( P < .01) only in patients positive for EASF. CONCLUSIONS: The levels of EASF, E 2 , and FSH may act synergistically in the development of ovarian follicles.