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Predominant Production of Amniotic Interleukin‐1α in Cases With Premature Rupture of the Membranes
Author(s) -
HIRANO KOHKI,
DAITOH TOSHIFUMI,
KAMADA MASAHARU,
MAEDA NOBUHIKO,
OHMOTO YASUKAZU,
AONO TOSHIHIRO
Publication year - 1993
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/j.1600-0897.1993.tb00582.x
Subject(s) - prom , amniotic fluid , umbilical cord , placenta , medicine , amnion , andrology , premature rupture of membranes , immunohistochemistry , fetus , chorionic villi , fetal membrane , amniotic sac , pregnancy , obstetrics , pathology , immunology , biology , genetics
PROBLEM: The physiological significances of the two known subtypes of interleukin‐1 (IL‐1) in amniotic fluid (AF) were examined by measurements of their concentrations and detection of their location. METHOD: Each IL‐1 subtype in AF collected from 61 patients were examined by newly developed enzyme‐linked immunosorbent assay with high sensitivity. Placenta and umbilical cord from 17 patients were examined immunohistochemically for localization of IL‐1. RESULTS: Different types of IL‐1 were predominant in AF; the mean (± SE) concentration of IL‐1β (412.8 ± 105.4pg/ml) was significantly higher ( P <0.01) than that of IL‐1α(116.4± 26.7 pg/ml) in cases with spontaneous vaginal delivery, whereas the concentration of IL‐1α (243.1 ±81.4pg/ml) was significantly higher(P<0.01)than that of IL‐1β (139.7±59.4pg/ml) in cases with premature rupture of the membranes (PROM) without intrauterine infection. In these cases, immunohistochemical studies revealed the presence of IL‐1α in the trophoblastic layer of the placenta and epithelium of the umbilical cord. CONCLUSIONS: Amniotic IL‐1α was detected predominantly in cases with PROM, and it is thought to be produced in placenta and fetal skin keratinocyte.