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Polymorphism of the Pa Gene
Author(s) -
VISHTEH ARASH G.,
KUNZ HEINZ W.,
HASSETT ANDREA L. CORTESE,
GILL THOMAS J.
Publication year - 1992
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/j.1600-0897.1992.tb00762.x
Subject(s) - restriction fragment length polymorphism , biology , allele , haplotype , microbiology and biotechnology , gene , restriction enzyme , genetics , dna , polymorphism (computer science) , antibody , strain (injury) , gene expression , genotype , anatomy
PROBLEM: This study was undertaken to identify the number of alleles of the Pa gene at the DNA level and to correlate the presence of the different alleles with the ability of a strain to elicit an anti‐Pa antibody response when mated with a WF female. The Pa gene is present in both Pa + and Pa − strains of rats, but it has unique restriction fragment length polymorphisms (RFLP) in the two types of strains: 1.7 kb in Pa − and 1.8 kb in Pa + Xbal digests using a probe derived from the Pa gene. RESULTS: Examination of DNA from a variety of strains using different enzymes showed that there were characteristic RFLP patterns for Pa + and Pa − strains. Strains of the b haplotype, however, had an intermediate RFLP pattern, and all of these strains had a relatively low level of reactivity with anti‐Pa antibody. CONCLUSIONS: Thus, there are three alleles of the Pa gene based on their level of expression: Pa a , high; Pa b , low; and Pa − , none.