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Effect of Labor on Lymphocyte Subsets in Full‐Term Neonates
Author(s) -
SAMELSON RENEE,
LARKEY DEBORAH M.,
AMANKWAH K.S.,
MCCONNACHIE PETER
Publication year - 1992
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/j.1600-0897.1992.tb00761.x
Subject(s) - cd16 , lymphocyte , flow cytometry , cd3 , immunology , umbilical cord , cord blood , natural killer cell , t lymphocyte , monoclonal antibody , medicine , peripheral blood lymphocyte , full term , immunofluorescence , andrology , immune system , cd8 , biology , pregnancy , antibody , cytotoxic t cell , in vitro , biochemistry , genetics
PROBLEM: To determine the effect of labor on lymphocyte subsets in full‐term neonates. Method of Study . Cord blood obtained at delivery from full‐term neonates, six born vaginally and six born by elective Cesarean section, was analyzed for lymphocyte subsets. Monoclonal antibodies, immunofluorescence, and flow cytometry were utilized to determine the lymphocyte phenotype frequencies in these neonates. These frequencies were compared by mode of delivery and to adult peripheral blood reference ranges using a two‐tailed Student's t‐test, P <0.05. RESULTS: A profile of significantly elevated T (CD2, CD3) and helper cells (CD4) and depressed Natural Killer cells (CD16, CD56) is characteristic of term Cesarean section neonates. Significantly depressed frequency of T cells (CD2, CD3) and helper T cells (CD4) and elevated Natural Killer cells (CD16, CD56) is characteristic of vaginally delivered neonates CONCLUSIONS: The mode of delivery affects the lymphocyte subset frequencies in full‐term neonates.