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Evaluation of a Monoclonal Antibody, BC‐1, Which Identifies an Antigen Expressed on the Surface Membrane of Human Extravillous Trophoblast
Author(s) -
LOKE Y.W.,
HSI B.L.,
BULMER JUDITH N.,
GRIVAUX CATHERINE,
HAWLEY SASHA,
GARDNER LUCY,
KING ASHLEY,
CARTER N. P.
Publication year - 1992
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/j.1600-0897.1992.tb00727.x
Subject(s) - cytotrophoblast , trophoblast , antigen , monoclonal antibody , biology , microbiology and biotechnology , placenta , immunology , chemistry , antibody , genetics , pregnancy , fetus
This paper describes a new Mab BC‐1 which is directed at a cell surface antigen expressed by extravillous cytotrophoblast and not by villous cytotrophoblast, so it is useful for distinguishing between the two populations. The antigen recognised by BC‐1 is trypsin‐resistant, which allows it to be used for flow cytometric analysis of living trophoblast isolated by enzymic disaggregation. However, BC‐1 is not trophoblast‐specific but cross‐reacts with some other tissues, in particular endothelial cells and peripheral blood monocytes. The nature of this antigen has not been established.