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A Possible Mechanism of Immunotherapy for Patients With Recurrent Spontaneous Abortions
Author(s) -
SUGI TOSHITAKA,
MAKINO TSUNEHISA,
MARUYAMA TETSUO,
KIM WOO KYOON,
IIZUKA RIHACHI
Publication year - 1991
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/j.1600-0897.1991.tb01092.x
Subject(s) - immunotherapy , immunology , blocking antibody , antibody , lymphoblast , immunosuppression , mixed lymphocyte reaction , immune system , medicine , active immunotherapy , cytotoxic t cell , t lymphocyte , t cell , biology , cell culture , biochemistry , in vitro , genetics
The mechanism of the beneficial effect of immunotherapy for human reproductive wastage remains to be elucidated. Induction of blocking antibodies such as anti‐HLA class II antibodies and anti‐idiotypic antibodies was investigated as the mechanism of specific immunosuppression in pregnancy. We reported the changes in the mixed lymphocyte reaction (MLR), T‐cell subsets, and generation of anti‐idiotypic antibodies after immunotherapy compared to before immunotherapy. MLR was significantly ( P < 0.001) inhibited after the immunization. The mean inhibition rate was 50.2%, suggesting that MLR blocking antibodies were induced by immunotherapy. Binding of autoantibodies to alloactivated maternal lymphoblasts against the paternal lymphocytes was detected in postimmunization cases in two‐color flow‐cytometric experiments. This suggests that anti‐idiotypic antibodies were induced by the immunotherapy. The percentage of cytotoxic T‐cells was significantly decreased ( P < 0.05) and the percentage of suppressor T‐cells was significantly increased ( P < 0.01) after the immunotherapy, suggesting that a cell‐mediated immune response was induced by the immunotherapy.