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Lymphocyte Subsets in the Lymph Nodes of Rats With Autoimmune Orchitis
Author(s) -
DONCEL GUSTAVO F.,
LUSTIG LIVIA
Publication year - 1991
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/j.1600-0897.1991.tb01061.x
Subject(s) - orchitis , lymph , cd8 , lymphocyte , immunization , infiltration (hvac) , immunology , lymphocyte subsets , immune system , biology , t lymphocyte , andrology , endocrinology , medicine , pathology , physics , thermodynamics
We determined temporal variations of cell subpopulations in the immunization draining lymph nodes during the development of an experimental autoimmune orchitis (EAO) induced in Wistar rats. A phenotypic characterization of T cells and their subsets (CD4 + and CD8 + ), B, and Ia + cells was performed by immunofluorescent technique. At the end of the immunization period (30 days), rats injected with testicular homogenate plus adjuvants presented a considerable increase in absolute cell number but normal lymphocyte subset percentages. Testicular damage became evident at 50 days after the first immunization and increased its severity at 80 days: animals that developed EAO presented a lower number of CD8 + cells as compared with undamaged rats. This latter group showed a low CD4/CD8 ratio due to the high proportion of CD8 + cells, which could probably have a suppressor function. At 80 days massive testicular infiltration and decreased absolute cell number in lymph nodes suggest the possible migration of specific lymphocytes to the target organ.