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Epstein‐Barr Virus Activating Principle in Husbands' Semen of Cervical Cancer Patients
Author(s) -
IDA KENJI,
TOKUDA HARUKUNI,
KANAOKA TOSHIO,
KANZAKI HIDEHARU,
NODA YOICHI,
YOSHIDA OSAMU,
ITO YOHEI,
MORI TAKAHIDE
Publication year - 1991
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/j.1600-0897.1991.tb00978.x
Subject(s) - semen , raji cell , cervical cancer , epstein–barr virus , biology , virus , in vitro , immunology , lymphoblast , antigen , virology , andrology , microbiology and biotechnology , cancer research , cancer , cell culture , medicine , genetics
A short‐term in vitro assay for detecting tumor promotor activity has been cultivated utilizing the induction of Epstein‐Barr virus early antigen (EBV‐EA) in EBV genome‐carrying human lymphoblastoid cell line (Raji cell) system. By using this system, we found that some of human semen samples possessed a marked capacity to induce EBV‐EA, suggesting possible tumor promotor activity of semen. In the present study, 60 semen samples obtained from husbands of cervical cancer patients and 90 control samples were incubated with Raji cells for 48 h, and EBV‐EA‐expressing cells detected by indirect immunofluorescence were counted. The percentages of EBV‐EA‐positive cells treated with semen of cervical cancer patients' husbands (8.17±5.43%) were significantly higher than those of controls (5.23 ± 5.91%). Since most of EBV‐EA expressing effect on Raji cells show overlapping with tumor promotor activities, our present results implies the circumstantial association of seminal EBV‐EA inducing activity with occurrence of human cervical cancer.