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Effect of Human Seminal Plasma on the Lytic Activity of Natural Killer Cells and Presumptive Identification of Participant Macromolecules
Author(s) -
ABLIN RICHARD J.,
BARTKUS JOHN M.,
POLGÁR JANOS
Publication year - 1990
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/j.1600-0897.1990.tb00689.x
Subject(s) - lytic cycle , in vitro , effector , macromolecule , immunosuppression , biology , immunology , microbiology and biotechnology , natural killer cell , chemistry , biochemistry , cytotoxicity , virus
Using the lytic activity of natural killer (NK) cells as an in vitro parameter, the immunoregulatory properties of human seminal plasma (SeP1) and participant macromolecules have been investigated. Significant ( P < 0.05) suppression of NK cell activity by SeP1 and chromatographically separable fractions was demonstrated in association with high and low molecular weight (M r ) macromolecules. SePl suppression was retained after heating to 56°C for 30 min, and appeared to function at the level of the effector, rather than target cell. Physicochemical characterization of high and low M r fractions provided presumptive identification of the participation of transglutaminase and prostaglandins as the principal molecules contributory to SeP1 immunosuppression.