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Toward Regulation of Gonadal Function by a Synthetic Hybrid Molecule Composed of Gonadotropin and Fc Fragment of Immunoglobulin G
Author(s) -
JOHNSON G.A.,
WILKEN C.,
KIRK E.A.,
BELDEN E.L.,
MURDOCH W.J.
Publication year - 1990
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/j.1600-0897.1990.tb00663.x
Subject(s) - human chorionic gonadotropin , gonadotropin , receptor , medicine , antibody , endocrinology , testosterone propionate , luteinizing hormone , in vitro , complement fixation test , biology , chemistry , hormone , immunology , biochemistry , androgen , serology
A conjugate of human chorionic gonadotropin (HCG) and Fc fragment of immunoglobulin G was prepared by covalent cross‐linking using the heterobifunctional reagent, N‐succini‐midyl 3‐(2‐pyridyldithio) propionate. Mouse Leydig tumor cells expressing receptors for luteinizing hormone were specifically lysed in vitro as a consequence of complement fixation via the Fc component of the hybrid molecule. Furthermore, administration of HCG‐Fc to rams caused an acute depression in circulatory testosterone. This novel concept of targeted inhibition of gonadal function could prove to have future applications in control of reproductive processes.