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Mononuclear Cell Subpopulations in Human Follicular Fluid From Stimulated Cycles
Author(s) -
CASTILLA JOSE A.,
SAMPALO ALMUDENA,
MOLINA RAMON,
SAMANIEGO FRANCISCO,
MOZAS JUAN,
VERGARA FRANCISCO,
GARRIDO FEDERICO,
HERRUZO ALFONSO J.
Publication year - 1990
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/j.1600-0897.1990.tb00655.x
Subject(s) - follicular fluid , peripheral blood mononuclear cell , cd14 , follicular phase , cd8 , cd3 , lymphocyte , biology , endocrinology , medicine , andrology , immunology , ovary , t lymphocyte , peripheral blood lymphocyte , in vitro fertilisation , immune system , in vitro , oocyte , embryo , microbiology and biotechnology , biochemistry
The study of lymphocyte subsets from human follicular fluid (FF) provides an opportunity to evaluate immunological features of the ovary. We investigated the mononuclear cell subsets in FF and peripheral blood obtained at the time of laparoscopy from ten in vitro fertilization (IVF) patients. Midcycle nonpregnant peripheral blood was used as the control. A marked increase in the proportion of monocytes (CD14 + ) was observed in FF. Although FF was enriched with CD8 + lymphocytes, a decrease in the proportion of CD4 + lymphocytes was observed. “Memory” T cells in FF, identified by the CD4 + CD45R − phenotype, predominated over “naive” T cells (CD4 + CD45R + ) at a ratio of 2:1, which differs from the ratio yielded by control blood samples (1:1). The percentage of activated T cells (CD3 + HLA‐DR + cell) increased significantly in FF. When lymphocyte subsets were studied in the peripheral blood of IVF patients, changes similar to but less significant than those in FF were found. These data support the concept that lymphocytes play an important role in ovarian physiology.