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Effects of Mouse Testicular Extract on Immunocompetent Cells
Author(s) -
EMOTO MASASHI,
YAGYU YOSHIHIKO,
NISHIKAWA FUMIKO,
KATSUI NORIAKI,
KITA EIJI,
KASHIBA SHUZO
Publication year - 1989
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/j.1600-0897.1989.tb01003.x
Subject(s) - cytostasis , concanavalin a , biology , seminiferous tubule , macrophage , in vitro , antigen , microbiology and biotechnology , andrology , endocrinology , medicine , immunology , chemistry , cytotoxicity , biochemistry , sertoli cell , spermatogenesis
We investigated mouse testicular extract (TE) to clarify its biological functions in reproductive immunity. TE, at concentrations of 50–300 μg/ml, enhanced macrophage activities of spreading, glucose consumption, and cytostasis against a susceptible tumor cell line. On the other hand, TE inhibited concanavalin A (Con A)‐induced T‐cell blastogenesis in the dose range of 10–600 μg/ml. To elucidate the origin of TE, W/W v mice, which genetically lack germ cells, were used. TE obtained from W/W v mice enhanced the spreadability of macrophages and inhibited Con A‐induced blastogenesis of T cells. The enhancement of macrophage spreading was only achieved by the interstitial fluid (IF), while the suppression of Con A‐induced T‐cell responses was detected in seminiferous tubule fluid (STF) as well as in IF. TE did not affect listerial antigen‐specific responses of lymphocytes in vitro. These results suggest that TE has the capacity to regulate the biological responses associated with reproduction.