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In Vitro Sensitivity of Candida (Torulopsis) glabrata to Clotrimazole
Author(s) -
FAGNANT JOAN E.,
CLARK RICHARD B.,
MONIF GILLES R.G.
Publication year - 1989
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/j.1600-0897.1989.tb00545.x
Subject(s) - clotrimazole , microbiology and biotechnology , candida albicans , candida glabrata , biology , corpus albicans , agar dilution , agar , mycosis , in vitro , minimum inhibitory concentration , bacteria , antimicrobial , antifungal , immunology , biochemistry , genetics
ABSTRACT: Vulvovaginitis caused by Candida (Torulopsis) glabrata is often refractory to intravaginal imidazole therapy. Clotrimazole achieves its fungistatic activity for Candida albicans and C. glabrata by inhibiting different steps in intermediary cell metabolism. For C. glabrata , alkylation precedes dimethylation. The possibility that this altered sequence might account for the relative therapeutic nonresponsiveness was studied by determining comparative minimal inhibitory concentrations (MICs) of clotrimazole. In vitro analyses of ten strains of C. glabrata and 30 control strains of C. albicans performed using both agar and broth dilution tests revealed that fourfold lower MICs were consistently demonstrable with C. glabrata , irrespective of inoculum size. The data suggest that clinical difficulties encountered in the therapy of torulopsis vulvovaginitis probably represent the inability of intravaginal medication to eradicate urethral/urinary bladder colonization and subsequent reinfection rather than true therapeutic failures.