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Simple Radioimmunobinding Assay for Quantitation of Sperm Antibodies of IgG Immunoglobulin Class
Author(s) -
CLARKE GARY N.
Publication year - 1988
Publication title -
american journal of reproductive immunology and microbiology
Language(s) - English
Resource type - Journals
eISSN - 1600-0897
pISSN - 8755-8920
DOI - 10.1111/j.1600-0897.1988.tb00224.x
Subject(s) - antibody , sperm , immunoglobulin g , serial dilution , chemistry , immunology , microbiology and biotechnology , immunoglobulin e , biology , medicine , andrology , pathology , alternative medicine
The immunobead test (IBT) is an excellent test for initial sperm antibody screening and evaluation but is impractical when used to quantitate antibody levels using a twofold dilution series. The aim of this study, therefore, was to develop a relatively simple radioimmunobinding assay that would allow quantitation of sperm antibodies of IgG immunoglobulin class, which predominate in male sera. [ 125 I]‐Protein G was chosen as the radioligand because it binds to all IgG subclasses, but not to IgA or IgM. The results of the investigation indicate that the [ 125 I]‐protein G assay (PGA) allows efficient quantitation of sperm antibodies, as evidenced by a highly significant ( P< .0001) correlation (Spearman's, Rs = 0.94) between the PGA results and IgG‐IBT titres.