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Trophoblast Antigens in Human Seminal Plasma
Author(s) -
KAJINO TORU,
TORRY DONALD S.,
McINTYRE JOHN A.,
FAULK W. PAGE
Publication year - 1988
Publication title -
american journal of reproductive immunology and microbiology
Language(s) - English
Resource type - Journals
eISSN - 1600-0897
pISSN - 8755-8920
DOI - 10.1111/j.1600-0897.1988.tb00209.x
Subject(s) - antigen , trophoblast , antiserum , antibody , immunology , microbiology and biotechnology , blot , biology , chemistry , placenta , biochemistry , fetus , genetics , gene , pregnancy
Secondary recurrent spontaneous (2°) aborters manifest persistent IgG, which show differential cytotoxicity patterns with lymphocytes from many donors. These are non‐HLA‐directed antibodies, which react allotypically with both trophoblast and lymphocytes. The antigens they recognize are designated trophoblast‐lymphocyte crossreactive (TLX) antigens. Xenogeneic anti‐TLX sera were studied with the use of enzyme‐linked‐immunosorbent (ELISA) and immunochemical assays to determine the TLX status of seminal plasma. The results showed 1) allotypic TLX antigens are present in seminal plasma; 2) seminal plasma TLX antigens may be membrane associated; 3) by immuno‐blotting, the molecular weights of antigens reactive with TLX antisera are 15, 22, 28, 33 kD and a smear between 180 and 340 kd; 4) by isoelectric focusing, TLX antigens show pI 4.0, 5.35, 5.9, 6.5, 6.8, and 7.2. Allotypic seminal plasma TLX antigens may provide the antigenic stimuli for persistent maternal humoral immunity.