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Natural Killer Cytotoxicity and Antibody‐Dependent Cell Cytotoxicity to Herpes Simplex Virus‐Infected Target Cells in Murine Pregnancy
Author(s) -
LOFTIN KARIN C.,
GONIK BERNARD,
KUMARAN PRIYA
Publication year - 1988
Publication title -
american journal of reproductive immunology and microbiology
Language(s) - English
Resource type - Journals
eISSN - 1600-0897
pISSN - 8755-8920
DOI - 10.1111/j.1600-0897.1988.tb00202.x
Subject(s) - antibody dependent cell mediated cytotoxicity , herpes simplex virus , cytotoxicity , virus , virology , biology , antibody , natural killer cell , immunology , herpesviridae , antiserum , microbiology and biotechnology , in vitro , viral disease , biochemistry
In vitro natural killer cytotoxicity (NKC) and antibody‐dependent cell cytotoxicity (ADCC) activity against herpes simplex virus (HSV)‐infected cells were evaluated in a pregnant murine model (C 57 B1 6 inbred strain). Virgin (n = 16) and pregnant (late gestation) mice (n = 15) were infected intraperitoneally with HSV, type 1. After 18 hr, a 0.5‐ml aliquot of the peritoneal wash was frozen for virus plaque assay, and the cells were cultured in the 51 chromium release assay for NKC and ADCC. %NKC (mean ± S.E.) to HSV‐infected targets was significantly suppressed ( P < 0.05) in pregnant mice, 10.3% ± 1.9, compared to that of virgin mice, 32.5% ± 2.5. This suppression was abrogated with HSV‐specific antisera (%ADCC); 53.9% ± 4.4 (pregnant) compared to 49.1% ± 3.6 (virgin). The diminished NKC activity in pregnant mice was reflected in an increased mean number of virus particles in the peritoneal wash, 266 + 66 PFU/ml, compared to 38 ± 11 PFU/ml in virgin mice (P < 0.05). We concluded that NKC, but not ADCC, to HSV‐infected targets was suppressed and that HSV elimination was impaired in pregnant mice.