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Immunoregulatory Factor Released From a Cell Line Derived From Human Decidual Tissue
Author(s) -
TATSUMI KENICHI,
MORI TSUNEATSU,
MORI ETSUKO,
KANZAKI HIDEHARU,
MORI TAKAHIDE
Publication year - 1987
Publication title -
american journal of reproductive immunology and microbiology
Language(s) - English
Resource type - Journals
eISSN - 1600-0897
pISSN - 8755-8920
DOI - 10.1111/j.1600-0897.1987.tb00099.x
Subject(s) - cell culture , mixed lymphocyte reaction , decidual cells , cell , size exclusion chromatography , endocrinology , immunology , chemistry , biology , medicine , microbiology and biotechnology , t cell , biochemistry , immune system , pregnancy , fetus , genetics , placenta , enzyme
The culture supernatant of the TTK‐1 cell line, established from human decidual tissue, was found to contain a factor that strongly suppressed the mixed lymphocyte reaction (MLR). The mechanism of the MLR‐suppressive activity as well as the biochemical characterization of this factor was analyzed. The TTK‐1 supernatant suppressed the MLR much more strongly than the culture supernatants of the three other malignant cell lines examined. The molecular weight of this factor was estimated to be between 43 kilodaltons (kd) and 67 kd by gel filtration chromatography. The TTK‐1 supernatant also suppressed the proliferation of the interleukin 2 (IL‐2)‐dependent T cell lines, but did not suppress that of the IL‐2‐independent T cell lines, suggesting that the TTK‐1 supernatant inhibited the action of IL‐2 and subsequently suppressed the MLR. The fact that the TTK‐1 cell line originated from human decidual tissue might imply the important role of this factor in immunological fetomaternal balance.